The future of biodiversity is strongly linked with cities, and the people within cities. While urban growth threatens biodiversity, cities can also harbour a large variety of plant and animal species. In parallel, urban biodiversity also matters for people’s quality of life. Thus, timely challenges for urban social-ecological research and planning include understanding (1) the role of urban environments for species of conservation concern and (2) human-urban wildlife (including mammals, insects and plants) interactions in order to promote co-existence and sharing the urban landscape. This is essential information for inclusive management of biodiversity and human well-being in urban environments. In this session, we focus on synergism between urban biodiversity conservation and valuation of and interactions with urban nature by urban people in research, management and planning. We invite studies that deepen our understanding of intersections between urban biodiversity conservation and people’s daily life. Furthermore, we invite work that envisions pathways and tangible solutions towards managing biodiverse urban environments where positive human-wildlife experiences thrive.